PCB Prototype Board

I have been trying to move my circuit from the breadboard onto a piece of prototype board. It is a more secure and permanent solution than a breadboard, it would also give me more freedom on positioning the circuit. As I transfer the components onto the board, the range of load cell read by the microcontroller decreases, whereas the range at the output of the amplifier remains the same. I have tried to rearrange the layout, taken all components out and put them back onto the breadboard to see if any component is burnt during soldering. The reason for this change is unknown yet.